{"id":2665,"date":"2022-04-11T12:05:39","date_gmt":"2022-04-11T05:05:39","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/dnk-polimeraza-i-e-coli-fragment-klenova\/"},"modified":"2026-01-11T17:40:43","modified_gmt":"2026-01-11T10:40:43","slug":"dnk-polimeraza-i-e-coli-fragment-klenova","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/dnk-polimeraza-i-e-coli-fragment-klenova\/","title":{"rendered":"DNA polymerase I E.coli (Klenow Fragment)"},"content":{"rendered":"

DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3` -> 5` exonuclease activity, but has lost 5` -> 3` exonuclease activity.
Applications:
– dideoxy sequencing;
– polishing ends;
– second strand cDNA synthesis<\/p>\n

Source:<\/strong> Isolated from E.coli strain that carries the cloned DNA Polymerase I, Large (Klenow) Fragment gene.<\/p>\n

Unit definition:<\/strong> DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3` -> 5` exonuclease activity, but has lost 5` -> 3` exonuclease activity.
Applications:
– dideoxy sequencing;
– polishing ends;
– second strand cDNA synthesis<\/p>\n

Reaction buffer:<\/strong> SE-buffer Klenow fragment<\/p>\n

Storage conditions:<\/strong> 10 mM KH2<\/sub>PO4<\/sub> (pH 7.5); 50 mM KCl; 0.5 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.<\/p>\n

Quality control:<\/strong> The enzyme is purified free of contaminating endonucleases andexonucleases.
\n<\/p>","protected":false},"excerpt":{"rendered":"

DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3` -> 5` exonuclease activity, but has lost 5` -> 3` exonuclease activity. Applications: – dideoxy sequencing;– polishing ends;– second strand cDNA synthesis Source: Isolated from E.coli strain that carries the cloned DNA Polymerase I, […]<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[498],"product_tag":[],"class_list":{"0":"post-2665","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-enzymes","7":"pa_285b89be-ee4050614b","8":"pa_4ebaafd1-0ac48624f7","9":"pa_50b54da1-a5bfc9e079","10":"pa_concentration-13be3a710f","11":"pa_fasovka-65253e40ca","12":"pa_fasovka-658e880e51","13":"pa_package-d20caec3b4","14":"pa_package-5dbc98dcc9","16":"first","17":"instock","18":"taxable","19":"shipping-taxable","20":"purchasable","21":"product-type-variable","22":"berocket_lgv_grid","23":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2665","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2665"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2665"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2665"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2665"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}