Description: Exonuclease III (E. coli) catalyzes the stepwise removal of mononucleotides from 3`- hydroxyl termini of double stranded DNA. Exonuclease III activity depends partially on helical structure and displays sequence dependence (C > A = T > G). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications.
Applications:
– unidirectional nested deletions;
– site-directed mutagenesis;
– preparation of strand-specific probes;
– preparation of single-stranded substrates for dideoxy sequencing.<\/p>\n
Source:<\/strong> Isolated from E.coli.<\/p>\n Unit definition:<\/strong> Description: Exonuclease III (E. coli) catalyzes the stepwise removal of mononucleotides from 3`- hydroxyl termini of double stranded DNA. Exonuclease III activity depends partially on helical structure and displays sequence dependence (C > A = T > G). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications. Reaction buffer:<\/strong> SE-buffer Exonuclease III<\/p>\n Storage conditions:<\/strong> 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.5 mM EDTA; 1 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.<\/p>\n Quality control:<\/strong> <\/p>\n Notes:<\/strong> Description: Exonuclease III (E. coli) catalyzes the stepwise removal of mononucleotides from 3`- hydroxyl termini of double stranded DNA. Exonuclease III activity depends partially on helical structure and displays sequence dependence (C > A = T > G). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions […]<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[498],"product_tag":[],"class_list":{"0":"post-2679","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-enzymes","7":"pa_285b89be-8b68bed5b2","8":"pa_4ebaafd1-06fb4cc885","9":"pa_50b54da1-a5bfc9e079","10":"pa_concentration-e43099456c","11":"pa_fasovka-589257efd5","12":"pa_fasovka-cf16014404","13":"pa_package-d20caec3b4","14":"pa_package-5dbc98dcc9","16":"first","17":"instock","18":"taxable","19":"shipping-taxable","20":"purchasable","21":"product-type-variable","22":"berocket_lgv_grid","23":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2679","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2679"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2679"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2679"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2679"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Applications:
– unidirectional nested deletions;
– site-directed mutagenesis;
– preparation of strand-specific probes;
– preparation of single-stranded substrates for dideoxy sequencing.<\/p>\n
\n<\/p>","protected":false},"excerpt":{"rendered":"