{"id":2682,"date":"2022-04-11T12:05:59","date_gmt":"2022-04-11T05:05:59","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/endonukleaza-i\/"},"modified":"2026-01-11T17:40:50","modified_gmt":"2026-01-11T10:40:50","slug":"endonukleaza-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/endonukleaza-i\/","title":{"rendered":"Endonuclease I"},"content":{"rendered":"<p>Endonuclease I hydrolyzes double- and single-stranded nucleic acids to oligonucleotides of 3-5 nucleotide lenghts with 5` &#8211; terminal phosphates.<br \/>\nApplication: DNA and RNA degradation<\/p>\n<p><strong>Source:<\/strong> Isolated from Proteus vulgaris 84.<\/p>\n<p><strong>Unit definition:<\/strong> One unit is defined as the amount of enzyme required to cleave 1 \u03bcg of the indicated double-stranded oligonucleotide in 30 min at 37\u00b0C in a total reaction volume of 20 \u03bcl.<br \/>\nApplication: DNA and RNA degradation<\/p>\n<p><strong>Assayed on:<\/strong><br \/>\nDouble-stranded oligonucleotide<br \/>\n5\u2019-AGCAAATATTGCTCGATATC-3\u2019<br \/>\n3\u2019-TCGTTTATAACGAGCTATAG-5\u2019<\/p>\n<p><strong>Reaction buffer:<\/strong> SE-buffer Endonuclease I<\/p>\n<p><strong>Storage conditions:<\/strong> 10 mM Tris-HCl (pH 7.4); 250 mM NaCl; 0,2 mM EDTA; 100 \u03bcg\/ml BSA; 7 mM 2-mercaptoetanol ; 50% glycerol. Store at -20\u00b0C.<\/p>\n<p><strong>Quality control:<\/strong> 10 units of Endonuclease I is incubated with 20 ng of a single or double-stranded synthetic oligonucleotide in a 10 \u03bcl reaction mixture with the SE Endonuclease I Buffer for 3 hours at 37\u00b0C.Phosphatase contamination is determined by the loss of radioactivity from original 5` <sup>32<\/sup>P-labeled oligonucleotide or products of <sup>32<\/sup>P-labeled oligonucleotide digestion with Endonucleases I as determined by denaturating polyacrylamide gel electrophresis and subsequent autoradiography.<\/p>\n<table>\n<tbody>\n<tr align=\"center\">\n<td>Runs:<br \/>\n1. double-stranded synthetic oligonucleotide 5`p*GTGCATGGCGCCTATGCG3`\/ 3`CACGTACCGCGGATACGC5`<br \/>\n2. double-stranded synthetic oligonucleotide 5`p*GTGCATGGCGCCTATGCG3`\/ 3`CACGTACCGCGGATACGC5` plus Endonuclease I<br \/>\n3. single-stranded synthetic oligonucleotide 5`p*GTGCATGGCGCCTATGCG3`plus Endonuclease I<br \/>\n4. double-stranded synthetic oligonucleotide 5`p*GTGCATGGCGCCTATGCG3`\/ 3`CACGTACCGCGGATACGC5` plus endonucleases Fai I (recognition sequence YA^TR)<br \/>\n5. single-stranded synthetic oligonucleotide 5`p*GTGCATGGCGCCTATGCG3`<br \/>\n6. single-stranded synthetic oligonucleotide 5`p*GTGCATGGCGCCTATGCG3`plus Alkaline Phosphatase<br \/>\nlabeled strand is marked by a symbol &lt;*&gt;<\/td>\n<td align=\"center\"><img decoding=\"async\" src=\"\/wp-content\/uploads\/img\/endonuclease-i.jpg\" \/><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>Endonuclease I hydrolyzes double- and single-stranded nucleic acids to oligonucleotides of 3-5 nucleotide lenghts with 5` &#8211; terminal phosphates. Application: DNA and RNA degradation Source: Isolated from Proteus vulgaris 84. Unit definition: One unit is defined as the amount of enzyme required to cleave 1 \u03bcg of the indicated double-stranded oligonucleotide in 30 min at [&hellip;]<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[498],"product_tag":[],"class_list":{"0":"post-2682","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-enzymes","7":"pa_285b89be-a9c1a57dfa","8":"pa_4ebaafd1-a22d307cf4","9":"pa_50b54da1-a5bfc9e079","10":"pa_concentration-d1b2b8c121","11":"pa_fasovka-acae4125ae","12":"pa_fasovka-5779c21a55","13":"pa_package-d20caec3b4","14":"pa_package-5dbc98dcc9","16":"first","17":"instock","18":"taxable","19":"shipping-taxable","20":"purchasable","21":"product-type-variable","22":"berocket_lgv_grid","23":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2682","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2682"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2682"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2682"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2682"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}