{"id":2697,"date":"2022-04-11T12:05:59","date_gmt":"2022-04-11T05:05:59","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/t7-rnk-polimeraza\/"},"modified":"2026-01-26T16:35:30","modified_gmt":"2026-01-26T09:35:30","slug":"t7-rnk-polimeraza","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/t7-rnk-polimeraza\/","title":{"rendered":"T7 RNA polymerase"},"content":{"rendered":"<p>T7 RNA Polymerase catalyzes the synthesis of RNA in the 5` -&gt; 3` direction in the presence of a DNA template containing a T7 phage promoter.<br \/>Applications:<br \/>-Radiolabeled RNA probe preparation<br \/>-RNA generation for in vitro translation<br \/>-RNA generation for studies of RNA structure,processing and catalysis<\/p>\n<p><strong>Source:<\/strong> Isolated from E.coli strain that carries the cloned T7 gene I<\/p>\n<p><strong>Unit definition:<\/strong> T7 RNA Polymerase catalyzes the synthesis of RNA in the 5` -&gt; 3` direction in the presence of a DNA template containing a T7 phage promoter.<br \/>Applications:<br \/>-Radiolabeled RNA probe preparation<br \/>-RNA generation for in vitro translation<br \/>-RNA generation for studies of RNA structure,processing and catalysis<\/p>\n<p><strong>Reaction buffer:<\/strong> SE-buffer RNA polymerase T7<\/p>\n<p><strong>Storage conditions:<\/strong> 50 mM Tris &#8211; HCl (pH 7.5); 100 mM NaCl; 20 mM 2-mercaptoethanol; 1 mM EDTA; 0.1% Triton X-100; 50% glycerol.  Store at -20&#176;C.<\/p>\n<p><strong>Quality control:<\/strong> The  enzyme  is  purified  free  of  contaminating  endonucleases  andexonucleases.<br \/>\n<\/p>","protected":false},"excerpt":{"rendered":"<p>T7 RNA Polymerase catalyzes the synthesis of RNA in the 5` -&gt; 3` direction in the presence of a DNA template containing a T7 phage promoter.Applications:-Radiolabeled RNA probe preparation-RNA generation for in vitro translation-RNA generation for studies of RNA structure,processing and catalysis Source: Isolated from E.coli strain that carries the cloned T7 gene I Unit [&hellip;]<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[498],"product_tag":[],"class_list":{"0":"post-2697","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-enzymes","7":"pa_285b89be-956bc438f1","8":"pa_4ebaafd1-pa_4ebaafd_d66329-401","9":"pa_50b54da1-a5bfc9e079","10":"pa_concentration-682d1e9922","11":"pa_fasovka-589257efd5","12":"pa_fasovka-cf16014404","13":"pa_package-d20caec3b4","14":"pa_package-5dbc98dcc9","16":"first","17":"instock","18":"taxable","19":"shipping-taxable","20":"purchasable","21":"product-type-variable","22":"berocket_lgv_grid","23":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2697","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2697"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2697"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2697"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2697"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}