{"id":2718,"date":"2022-04-11T12:40:07","date_gmt":"2022-04-11T05:40:07","guid":{"rendered":"http:\/\/sibenzyme.com\/product\/m-hspa-i\/"},"modified":"2026-01-11T17:40:54","modified_gmt":"2026-01-11T10:40:54","slug":"m-hspa-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/m-hspa-i\/","title":{"rendered":"M.HspA I"},"content":{"rendered":"<p><\/p>\n<table border=\"1\" width=\"95%\" cellspacing=\"10\" cellpadding=\"6\">\n<tbody>\n<tr>\n<td><b>Recognition site<\/b><\/td>\n<td><center><span class=\"fixed\">G(5mC)GC<\/span><\/center><\/td>\n<\/tr>\n<tr>\n<td><b>Source<\/b><\/td>\n<td>An E.coli strain, that carries the cloned M.HspAI gene from Haemophilus species AI.<\/td>\n<\/tr>\n<tr>\n<td><b>Description<\/b><\/td>\n<td>M.HspAI Methyltransferase modifies the internal cytosine residue (C<sup>5<\/sup>) in the recognition sequence forming 5`-G(5mC)GC-3`\/3`-CG(5mC)G-5`.<\/td>\n<\/tr>\n<tr>\n<td><b>Unit definition<\/b><\/td>\n<td>One unit of the enzyme is the amount required to protect 1 \u03bcg of Lambda DNA in 1 hour at 37\u00b0C in a total reaction volume of 20\u03bcl against cleavage by HspAI restriction endonuclease.<\/td>\n<\/tr>\n<tr>\n<td><b>Reaction buffer<\/b><\/td>\n<td><b>SE-buffer B + SAM<\/b><\/td>\n<\/tr>\n<tr>\n<td><b>Optimal temperature<\/b><\/td>\n<td><center>37<sup>o<\/sup>C<\/center><\/td>\n<\/tr>\n<tr>\n<td><b>Storage conditions<\/b><\/td>\n<td>10 mM Tis-HCl (pH 7.4); 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 100 mM NaCl; 50% glycerol. Store at -20\u00b0C.<\/td>\n<\/tr>\n<tr>\n<td><b>Notes<\/b><\/td>\n<td>To obtain 100% activity, SAM should be added to the 1x reaction mix to a final concentration of 0,08 mM.<\/td>\n<\/tr>\n<tr>\n<td><b>MSDS:<\/b><\/td>\n<td align=\"center\"><a title=\"MSDS-file for SibEnzyme product\" href=\"http:\/\/sibenzyme.com\/wp-content\/uploads\/msds\/MSDS---M.HspA-I.pdf\">Download MSDS as PDF<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>DNA Methyltransferase M.HspA I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[513],"product_tag":[],"class_list":{"0":"post-2718","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-dna-methyltransferases","7":"pa_17a2f6f2-1c30f8da56","8":"pa_4ebaafd1-7db787f975","9":"pa_50b54da1-a5bfc9e079","10":"pa_concentration-257f7e4df5","11":"pa_fasovka-1606ccd187","12":"pa_site-0b126217fa","14":"first","15":"instock","16":"taxable","17":"shipping-taxable","18":"purchasable","19":"product-type-simple","20":"berocket_lgv_grid","21":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/2718","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=2718"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=2718"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=2718"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=2718"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}