{"id":4113,"date":"2024-10-21T17:28:16","date_gmt":"2024-10-21T10:28:16","guid":{"rendered":"https:\/\/sibenzyme.com\/?post_type=product&p=4113"},"modified":"2026-01-11T17:42:09","modified_gmt":"2026-01-11T10:42:09","slug":"pec-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/pec-i\/","title":{"rendered":"Pec I"},"content":{"rendered":"

Recognition site and hydrolysis position<\/strong>:<\/p>\n\n\n\n\n
TTATAA<\/td>\nTTA\u2191TAA<\/td>\n<\/tr>\n
AATATT<\/td>\nAAT\u2193ATT<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Paracoccus species 12
\nUnit definition:<\/strong>
\nOne unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of Lambda DNA in 1 hour at 37\u00b0C in a total reaction volume of 50 \u03bcl.
\nAssayed on:<\/strong>
\nLambda DNA
\nOptimal SE-buffer:<\/strong> SE-buffer Y
\nEnzyme activity (%)<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
50-75<\/td>\n75-100<\/td>\n10-25<\/td>\n25-50<\/td>\n100<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage conditions<\/strong>: 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 \u03bcg\/ml BSA; and 50% glycerol; Store at -20\u00b0C.
\nLigations<\/strong>:
\nAfter 10-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated and of these 95% can be recut.
\nNon-specific hydrolisis<\/strong>:
\nNo nonspecific activity was detected after incubation of 1 \u03bcg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37\u00b0C.
\nReagents Supplied with Enzyme
\n<\/b>10 X SE-buffer Y
\nMethylation sensitivity<\/strong>: not tested
\nNotes:<\/strong> The minimum number of units that resulted in complete digestion of 1 \u03bcg of substrate DNA in 16 hours is 0,25.<\/p>\n

<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Pec I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-4113","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf","8":"pa_4ebaafd1-44faaeefd3","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-3","12":"pa_fasovka-pa_fasovka_e7d4b7-15","13":"pa_fasovka-934","14":"pa_fasovka-pa_fasovka_e7d4b7-16","15":"pa_fasovka-3a4f8ebe60","16":"pa_obem-pa_obem_d1a3e6","17":"pa_obem-pa_obem_d1a3e6-1","18":"pa_package-d20caec3b4","19":"pa_package-5dbc98dcc9","20":"pa_site-eb09b8715f","21":"pa_variant-d2203cb123","22":"pa_variant-6f53bfe04e","24":"first","25":"instock","26":"featured","27":"taxable","28":"shipping-taxable","29":"purchasable","30":"product-type-variable","31":"berocket_lgv_grid","32":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/4113","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=4113"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=4113"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=4113"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=4113"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}