{"id":4260,"date":"2025-02-27T16:57:02","date_gmt":"2025-02-27T09:57:02","guid":{"rendered":"https:\/\/sibenzyme.com\/?post_type=product&p=4260"},"modified":"2026-01-11T17:42:10","modified_gmt":"2026-01-11T10:42:10","slug":"pse31-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/pse31-i\/","title":{"rendered":"Pse31 I"},"content":{"rendered":"

Recognition Site and Hydrolysis Position<\/strong>:<\/p>\n\n\n\n\n
GGTCTC(N)1<\/sub><\/td>\nGGTCTC(N)1<\/sub>\u2191<\/td>\n<\/tr>\n
CCAGAG(N)5<\/sub><\/td>\nCCAGAG(N)5<\/sub>\u2193<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Source:<\/strong> Peribacillus species 31
\nDefinition of Activity Unit:<\/strong>
\nOne unit of activity is defined as the amount of enzyme capable of hydrolyzing 1 \u00b5g of T7 DNA in a 50 \u00b5L reaction mixture in SE buffer Y at 37\u00b0C for 1 hour.
\nSubstrate for Activity Determination:<\/strong>
\nT7 DNA
\nOptimal Buffer:<\/strong> SE buffer Y
\nEnzyme Activity in Various Buffers as a Percentage of Maximum<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
25-50<\/td>\n75-100<\/td>\n10-25<\/td>\n25-50<\/td>\n100<\/td>\n75<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage Conditions<\/strong>: 10 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 200 \u00b5g\/mL BSA, 7 mM 2-mercaptoethanol, 50% glycerol; Store at -20\u00b0C
\nLigase Compatibility<\/strong>:
\nAfter hydrolysis with a 10-fold excess of enzyme, more than 90% of DNA fragments are ligated by DNA ligase, and approximately 90% of them can be re-cleaved.
\nNon-Specific Hydrolysis<\/strong>:
\nThe pattern of specific hydrolysis remains unchanged when treating 1 \u00b5g of DNA with 20 units of enzyme for 16 hours at 37\u00b0C. BSA is not recommended for prolonged incubation.
\nMethylation Sensitivity<\/strong>: Blocked 90-95% when overlapping with mCG methylation: 5′-GGTCT(5mC)G-3′ and\/or 5′-GAGAC(5mC)G-3′.
\nSupplied with the Enzyme:<\/strong> 10 x SE Buffer Y, BSA (10 mg\/mL).
\nFor Turbo packaging, 10 X SE Buffer ROSE is included.<\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Pse31 I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[],"product_cat":[23],"product_tag":[],"class_list":{"0":"post-4260","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-re","7":"pa_285b89be-pa_285b89b_2d3edf","8":"pa_4ebaafd1-6d3b4203b8","9":"pa_50b54da1-pa_50b54da_6e490e","10":"pa_be110261-pa_be11026_383772-2","11":"pa_concentration-pa_concent_8cc665-3","12":"pa_fasovka-pa_fasovka_e7d4b7-15","13":"pa_fasovka-934","14":"pa_fasovka-pa_fasovka_e7d4b7-16","15":"pa_fasovka-3a4f8ebe60","16":"pa_obem-pa_obem_d1a3e6","17":"pa_obem-pa_obem_d1a3e6-1","18":"pa_package-d20caec3b4","19":"pa_package-5dbc98dcc9","20":"pa_site-d87708a7f1","21":"pa_variant-d2203cb123","22":"pa_variant-6f53bfe04e","23":"pa_-pa__3fb5ca-3","25":"first","26":"instock","27":"featured","28":"taxable","29":"shipping-taxable","30":"purchasable","31":"product-type-variable","32":"berocket_lgv_grid","33":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/4260","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=4260"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=4260"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=4260"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=4260"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}