{"id":4715,"date":"2025-11-19T14:51:55","date_gmt":"2025-11-19T07:51:55","guid":{"rendered":"https:\/\/sibenzyme.com\/?post_type=product&p=4715"},"modified":"2026-01-16T15:25:58","modified_gmt":"2026-01-16T08:25:58","slug":"bmo-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/bmo-i\/","title":{"rendered":"Bmo I"},"content":{"rendered":"

Recognition Site and Hydrolysis Position<\/strong>:<\/p>\n

\n
5'\u2026 GG\u25bc<\/sup>GWCCC \u20263'<\/div>\n
3'\u2026 CCCWG\u25b2<\/sub>GG \u20265'<\/div>\n <\/div>\n \n

Source:<\/strong> Brevundimonas mongoliensis 53<\/p>\n

Definition of Activity Unit: <\/strong>One unit is defined as the amount of enzyme required to digest 1 \u00b5g of T7 DNA in 1 hour at 37\u00b0C in a total reaction volume of 50 \u00b5l.<\/p>\n

Substrate for Activity Determination: <\/strong>T7 DNA<\/p>\n

Optimal Buffer:<\/strong> SE buffer W<\/p>\n

Enzyme Activity in Various Buffers as a Percentage of Maximum<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/td>\nROSE<\/td>\n<\/tr>\n
25-50<\/td>\n50-75<\/td>\n25-50<\/td>\n100<\/td>\n50-75<\/td>\n75<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage Conditions<\/strong>: 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol; Store at -20\u00b0C<\/mark><\/p>\n

Ligase Compatibility<\/strong>: After 3-fold overdigestion with Bmo I, >90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.<\/p>\n

Non-Specific Hydrolysis<\/strong>: A 50 \u00b5l reaction containing 1 \u00b5g of DNA and 4 units of enzyme incubated for 16 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour<\/p>\n

Oligonucleotide Assay<\/strong>: No detectable degradation of a single-stranded and double-stranded oligonucleotide was observed after incubation with 2 units of restriction endonuclease for 3 hours.<\/p>\n

Methylation Sensitivity<\/strong>: Blocked by overlapping CG-methylation GGGWCC(5mC)G<\/p>\n

Supplied with the Enzyme:<\/strong> 10x SE Buffer W<\/p>\n

Enzyme Properties<\/strong>: When using a buffer other than the optimal (supplied) SE-Buffer, it may be necessary to add more enzyme to achieve complete digestion.<\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Bmo I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[1659],"product_cat":[23],"product_tag":[969,970],"class_list":{"0":"post-4715","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-product_br_7fed80","7":"product_cat-re","8":"product_tag-bmoi","9":"product_tag-sandi","10":"pa_285b89be-pa_285b89b_2d3edf-2","11":"pa_4ebaafd1-1b96a03329","12":"pa_50b54da1-pa_50b54da_6e490e","13":"pa_be110261-pa_be11026_383772-2","14":"pa_concentration-pa_concent_8cc665-10","15":"pa_fasovka-pa_fasovka_e7d4b7-4","16":"pa_fasovka-3a4f8ebe60","17":"pa_obem-pa_obem_d1a3e6","18":"pa_obem-pa_obem_d1a3e6-1","19":"pa_package-d20caec3b4","20":"pa_package-5dbc98dcc9","21":"pa_site-9cee9e0118","22":"pa_variant-d2203cb123","23":"pa_-pa__3fb5ca-2","25":"first","26":"instock","27":"featured","28":"taxable","29":"shipping-taxable","30":"product-type-variable","31":"berocket_lgv_grid","32":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/4715","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=4715"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=4715"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=4715"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=4715"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}