{"id":4794,"date":"2025-12-25T14:05:16","date_gmt":"2025-12-25T07:05:16","guid":{"rendered":"https:\/\/sibenzyme.com\/?post_type=product&#038;p=4794"},"modified":"2026-09-07T16:37:14","modified_gmt":"2026-09-07T09:37:14","slug":"emis6-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/emis6-i\/","title":{"rendered":"EmiS6 I"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text css=&#8221;&#8221;]<div class=\"sbe-rebase-enzyme-card\"><table style=\"width:100%; border-collapse:collapse;\"><tbody>\n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Enzyme name<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">EmiS6 I<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Prototype<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Ksp632I<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">SKU<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">SE-E609<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Turbo version<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Not available<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">High-concentration version<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Not available<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Recognition site<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\"><div class=\"rs-box\"><div class=\"rs-line\">5'\u2026 CTCTTC(N)<sub class=\"rs-idx\">1<\/sub><sup class=\"rs-cut rs-cut-top\">\u25bc<\/sup> \u20263'<\/div><div class=\"rs-line\">3'\u2026 GAGAAG(N)<sub class=\"rs-idx\">4<\/sub><sub class=\"rs-cut rs-cut-bottom\">\u25b2<\/sub> \u20265'<\/div><\/div><\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Source<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Exiguobacterium mexicanum 6 Definition of Activity Unit One unit is defined as the amount of enzyme required to digest 1 \u00b5g of \u03bb DNA in 1 hour at 37\u00b0C in a total reaction volume of 50 \u00b5l. Substrate for Activity Determination: \u03bb DNA<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Optimal buffer<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25\u00b0C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Optimal temperature<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">37 \u00b0C<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Inactivation temperature<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">80 \u00b0C<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Buffer activity<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\"><style>\n.sbe-flat-act-wrap{\n    overflow-x:auto;\n    margin:2px 0;\n}\n.sbe-flat-act{\n    width:100%;\n    max-width:760px;\n    border-collapse:collapse;\n    table-layout:fixed;\n    font-size:14px;\n    line-height:1.25;\n    background:transparent;\n}\n.sbe-flat-act th,\n.sbe-flat-act td{\n    text-align:center;\n    padding:10px 8px;\n    border-right:1px solid #d6d6d6;\n}\n.sbe-flat-act th:last-child,\n.sbe-flat-act td:last-child{\n    border-right:none;\n}\n.sbe-flat-act thead th{\n    font-weight:700;\n    color:#1f2a44;\n}\n.sbe-flat-act tbody td{\n    color:#111;\n    background:#fff;\n    padding-top:11px;\n    padding-bottom:11px;\n    border-top:1px solid #d6d6d6;\n}\n.sbe-flat-act .buf-b{background:#8697e8;}\n.sbe-flat-act .buf-g{background:#88b68a;}\n.sbe-flat-act .buf-o{background:#efc97d;}\n.sbe-flat-act .buf-w{background:#ffffff;}\n.sbe-flat-act .buf-y{background:#e4e97f;}\n.sbe-flat-act .buf-rose{background:#e9c2cb;}\n@media (max-width:640px){\n    .sbe-flat-act{min-width:560px;}\n}\n<\/style><div class=\"sbe-flat-act-wrap\"><table class=\"sbe-flat-act\"><thead><tr><th class=\"buf-b\">B<\/th><th class=\"buf-g\">G<\/th><th class=\"buf-o\">O<\/th><th class=\"buf-w\">W<\/th><th class=\"buf-y\">Y<\/th><th class=\"buf-rose\">ROSE<\/th><\/tr><\/thead><tbody><tr><td>75<\/td><td>50<\/td><td>25<\/td><td>25<\/td><td>100<\/td><td>25<\/td><\/tr><\/tbody><\/table><\/div><\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Unit definition<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">One unit of the enzyme is the amount required to hydrolyze 1 \u03bcg of Lambda DNA in 1 hour at 37\u00b0C in a total reaction volume of 50 \u03bcl.<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Assayed on<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Lambda DNA<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Storage conditions<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 \u00b5g\/ml BSA, 50% glycerol Store at -20\u00b0C<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Ligation<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">After digestion with a 5-fold excess of enzyme, 95% of DNA fragments can be ligated by DNA ligase, and 80% of these can be recut.<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Nonspecific hydrolysis<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">A 50 \u00b5l reaction containing 1 \u00b5g of DNA and 5 units of enzyme incubated for 16 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour. Oligonucleotide Assay No detectable degradation of a single-stranded and double-stranded oligonucleotide was observed after incubation with 5 units of restriction endonuclease for 3 hours.<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Methylation sensitivity<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\"><\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Reagents supplied with enzyme<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">10x SE Buffer Y<\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Notes<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\"><\/td>\n            <\/tr>\n        \n            <tr>\n                <th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">References<\/th>\n                <td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">    <div class=\"sepub-list sepub-embedded\" data-sepub-language=\"en\">\r\n                <ol class=\"sepub-items\">\r\n                            <li class=\"sepub-item sepub-has-link\" data-search=\"m.a. abdurashitov, d.a. gonchar, v.a. chernukhin, v.s. dedkov, n.a. mikhnenkova, a.a. nikonova, s.kh. degtyarev the emis6i restriction endonuclease, the ksp632i isoisomer, recognizes the non-palindromic dna sequence 5\u2019-ctcttc(1\/4)-3\u2019. \/\/ dna-recognizing enzymes, vol. 2025(4), doi: 10.26213\/3034-4301.2025.7.4.002 (2025). (in russian) 2025\">\r\n                    <article>\r\n                        <div class=\"sepub-citation\">\r\n                                                                                    <span class=\"sepub-authors\">M.A. Abdurashitov, D.A. Gonchar, V.A. Chernukhin, V.S. Dedkov, N.A. Mikhnenkova, A.A. Nikonova, S.Kh. Degtyarev<\/span>\r\n                            <span class=\"sepub-title\">\r\n                                                                    <a href=\"https:\/\/dnape.online\/ru\/2025\/12\/emi-i\/\"\r\n                                       target=\"_blank\"\r\n                                       rel=\"noopener noreferrer\"\r\n                                                                           >The EmiS6I restriction endonuclease, the Ksp632I isoisomer, recognizes the non-palindromic DNA sequence 5\u2019-CTCTTC(1\/4)-3\u2019.<\/a>\r\n                                                                                                    <span class=\"sepub-bibliography\"> \/\/ DNA-Recognizing Enzymes, Vol. 2025(4), DOI: 10.26213\/3034-4301.2025.7.4.002 (2025). (In Russian)<\/span>\r\n                                                            <\/span>\r\n                                                            <time class=\"sepub-year\" datetime=\"2025\">2025<\/time>\r\n                                                    <\/div>\r\n                                            <\/article>\r\n                <\/li>\r\n                    <\/ol>\r\n        <p class=\"sepub-no-results\" hidden>Nothing found.<\/p>\r\n    <\/div>\r\n    <\/td>\n            <\/tr>\n        <\/tbody><\/table><\/div>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Restriction endonuclease EmiS6 I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[1659],"product_cat":[23],"product_tag":[1699,1700,1698,1701],"class_list":["post-4794","product","type-product","status-publish","has-post-thumbnail","product_brand-product_br_7fed80","product_cat-re","product_tag-product_ta_714772-1","product_tag-product_ta_714772-2","product_tag-product_ta_714772","product_tag-product_ta_714772-3","pa_285b89be-896200627f","pa_4ebaafd1-pa_4ebaafd_d66329-399","pa_50b54da1-pa_50b54da_6e490e","pa_be110261-pa_be11026_383772-3","pa_concentration-13be3a710f","pa_fasovka-65253e40ca","pa_fasovka-658e880e51","pa_obem-5ada2cbcd7","pa_obem-513434c781","pa_package-d20caec3b4","pa_package-5dbc98dcc9","pa_site-pa_site_aea581-1","pa_variant-d2203cb123","pa_-pa__3fb5ca-6","pa_-pa__3fb5ca-4","pa_-pa__3fb5ca-5","first","instock","featured","taxable","shipping-taxable","product-type-variable","berocket_lgv_grid","berocket_lgv_list_grid"],"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/4794","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=4794"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=4794"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=4794"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=4794"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}