{"id":4794,"date":"2025-12-25T14:05:16","date_gmt":"2025-12-25T07:05:16","guid":{"rendered":"https:\/\/sibenzyme.com\/?post_type=product&p=4794"},"modified":"2026-01-11T18:12:31","modified_gmt":"2026-01-11T11:12:31","slug":"emi-i","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/emi-i\/","title":{"rendered":"Emi I"},"content":{"rendered":"

Recognition Site and Hydrolysis Position<\/strong>:<\/p>\n

\n
5'\u2026 CTCTTC(N)1<\/sub>\u25bc<\/sup> \u20263'<\/div>\n
3'\u2026 GAGAAG(N)4<\/sub>\u25b2<\/sub> \u20265'<\/div>\n <\/div>\n \n

Source:<\/strong> Exiguobacterium mexicanum 6<\/p>\n

Definition of Activity Unit: <\/strong> One unit is defined as the amount of enzyme required to digest 1 \u00b5g of \u03bb DNA in 1 hour at 37\u00b0C in a total reaction volume of 50 \u00b5l.<\/p>\n

Substrate for Activity Determination: \u03bb <\/strong>DNA<\/p>\n

Optimal Buffer:<\/strong> SE buffer Y<\/p>\n

Enzyme Activity in Various Buffers as a Percentage of Maximum<\/strong>:<\/p>\n\n\n\n\n
B<\/td>\nG<\/td>\nO<\/td>\nW<\/td>\nY<\/strong><\/td>\nROSE<\/td>\n<\/tr>\n
75-100<\/td>\n50-75<\/td>\n25-50<\/td>\n25-50<\/td>\n100<\/strong><\/td>\n25<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Storage Conditions<\/strong>: 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 \u00b5g\/ml BSA, 50% glycerol; Store at -20\u00b0C<\/mark><\/p>\n

Ligase Compatibility<\/strong>: After 5-fold overdigestion with Emi I, 95% of the DNA fragments can be ligated with T4 DNA Ligase and 80% of those can be recut<\/p>\n

Non-Specific Hydrolysis<\/strong>: A 50 \u00b5l reaction containing 1 \u00b5g of DNA and 10 units of enzyme incubated for 16 hours
\nresulted in the same pattern of DNA bands as a reaction incubated for 1 hour.<\/p>\n

Oligonucleotide Assay<\/strong>: No detectable degradation of a single-stranded and double-stranded oligonucleotide was observed after incubation with 5 units of restriction endonuclease for 3 hours.<\/p>\n

Supplied with the Enzyme:<\/strong> 10x SE Buffer Y<\/p>\n

Enzyme Properties<\/strong>: When using a buffer other than the optimal (supplied) SE-Buffer, it may be necessary to add more enzyme to achieve complete digestion.<\/p>\n

Reference: <\/strong>Abdurashitov M.A., Gonchar D.A., Chernukhin V.A., Dedkov V.S., Mikhnenkova N.A., Nikonova A.A., Degtyarev S.Kh. (2025)\u00a0The restriction endonuclease EmiI, an isoschizomer of Ksp632I, recognizes the non-palindromic DNA sequence 5\u2032-CTCTTC(1\/4)-3\u2032.<\/a>\u00a0<\/em>DNA-Processing Enzymes, 2025, Vol. 4.<\/p>","protected":false},"excerpt":{"rendered":"

Restriction endonuclease Emi I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[1659],"product_cat":[23],"product_tag":[1699,1700,1698,1701],"class_list":{"0":"post-4794","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-product_br_7fed80","7":"product_cat-re","8":"product_tag-product_ta_714772-1","9":"product_tag-product_ta_714772-2","10":"product_tag-product_ta_714772","11":"product_tag-product_ta_714772-3","12":"pa_285b89be-896200627f","13":"pa_4ebaafd1-pa_4ebaafd_d66329-399","14":"pa_50b54da1-pa_50b54da_6e490e","15":"pa_be110261-pa_be11026_383772-3","16":"pa_concentration-13be3a710f","17":"pa_fasovka-65253e40ca","18":"pa_fasovka-658e880e51","19":"pa_obem-5ada2cbcd7","20":"pa_obem-513434c781","21":"pa_package-d20caec3b4","22":"pa_package-5dbc98dcc9","23":"pa_site-pa_site_aea581-1","24":"pa_variant-d2203cb123","25":"pa_-pa__3fb5ca-6","26":"pa_-pa__3fb5ca-4","27":"pa_-pa__3fb5ca-5","29":"first","30":"instock","31":"featured","32":"taxable","33":"shipping-taxable","34":"product-type-variable","35":"berocket_lgv_grid","36":"berocket_lgv_list_grid"},"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/4794","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=4794"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=4794"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=4794"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=4794"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}