{"id":5310,"date":"2026-10-15T13:49:58","date_gmt":"2026-10-15T06:49:58","guid":{"rendered":"https:\/\/sibenzyme.com\/?post_type=product&#038;p=5310"},"modified":"2026-10-15T14:04:21","modified_gmt":"2026-10-15T07:04:21","slug":"emis6-i-kopirovat","status":"publish","type":"product","link":"https:\/\/sibenzyme.com\/en\/product\/emis6-i-kopirovat\/","title":{"rendered":"Seg I"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text css=&#8221;&#8221;]<div class=\"sbe-rebase-enzyme-card\"><table style=\"width:100%; border-collapse:collapse;\"><tbody><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Enzyme name<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">SegI<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Prototype<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">FspAI<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Known analogs<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">FspAI<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">SKU<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">SE611<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Turbo version<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Not available<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">High-concentration version<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Not available<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Recognition site<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\"><div class=\"rs-box\"><div class=\"rs-line\">5'\u2026 RTGC<sup class=\"rs-cut rs-cut-top\">\u25bc<\/sup>GCAY \u20263'<\/div><div class=\"rs-line\">3'\u2026 YACG<sub class=\"rs-cut rs-cut-bottom\">\u25b2<\/sub>CGTR \u20265'<\/div><\/div><\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Source<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Sphingobium erigeronicola 46<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Optimal buffer<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25\u00b0C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Optimal temperature<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">37 \u00b0C<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Inactivation temperature<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">65 \u00b0C<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Buffer activity<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\"><style>\r\n.sbe-flat-act-wrap{\r\n    overflow-x:auto;\r\n    margin:2px 0;\r\n}\r\n.sbe-flat-act{\r\n    width:100%;\r\n    max-width:760px;\r\n    border-collapse:collapse;\r\n    table-layout:fixed;\r\n    font-size:14px;\r\n    line-height:1.25;\r\n    background:transparent;\r\n}\r\n.sbe-flat-act th,\r\n.sbe-flat-act td{\r\n    text-align:center;\r\n    padding:10px 8px;\r\n    border-right:1px solid #d6d6d6;\r\n}\r\n.sbe-flat-act th:last-child,\r\n.sbe-flat-act td:last-child{\r\n    border-right:none;\r\n}\r\n.sbe-flat-act thead th{\r\n    font-weight:700;\r\n    color:#1f2a44;\r\n}\r\n.sbe-flat-act tbody td{\r\n    color:#111;\r\n    background:#fff;\r\n    padding-top:11px;\r\n    padding-bottom:11px;\r\n    border-top:1px solid #d6d6d6;\r\n}\r\n.sbe-flat-act .buf-b{background:#8697e8;}\r\n.sbe-flat-act .buf-g{background:#88b68a;}\r\n.sbe-flat-act .buf-o{background:#efc97d;}\r\n.sbe-flat-act .buf-w{background:#ffffff;}\r\n.sbe-flat-act .buf-y{background:#e4e97f;}\r\n.sbe-flat-act .buf-rose{background:#e9c2cb;}\r\n@media (max-width:640px){\r\n    .sbe-flat-act{min-width:560px;}\r\n}\r\n<\/style><div class=\"sbe-flat-act-wrap\"><table class=\"sbe-flat-act\"><thead><tr><th class=\"buf-b\">B<\/th><th class=\"buf-g\">G<\/th><th class=\"buf-o\">O<\/th><th class=\"buf-w\">W<\/th><th class=\"buf-y\">Y<\/th><th class=\"buf-rose\">ROSE<\/th><\/tr><\/thead><tbody><tr><td>10-25<\/td><td>25-50<\/td><td>50-75<\/td><td>100<\/td><td>50-75<\/td><td>100<\/td><\/tr><\/tbody><\/table><\/div><\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Unit definition<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">One unit of activity is defined as the amount of enzyme required to digest 1 \u00b5g of bacteriophage T7 DNA in 50 \u00b5l of reaction mixture containing SE-buffer W at 37\u00b0C for 1 hour.<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Assayed on<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Bacteriophage T7 DNA<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Storage conditions<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">10 mM Tris-HCl (pH 7.6), 200 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 50% glycerol. Store at \u221220\u00b0C.<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Ligation<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">After digestion with a 3-fold excess of enzyme, DNA fragments are poorly ligated by T4 DNA ligase. Ligation efficiency exceeding 95% is achieved in the presence of 10% PEG.<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Nonspecific hydrolysis<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">The specific DNA digestion pattern remains unchanged after incubation of 1 \u00b5g DNA with 6 units of enzyme for 16 hours at 37\u00b0C. No nonspecific hydrolysis of single- or double-stranded oligonucleotides is observed after incubation with 3 units of enzyme for 3 hours.<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">Reagents supplied with enzyme<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">10\u00d7 SE-buffer W<\/td><\/tr><tr><th style=\"text-align:left; width:260px; padding:10px; border-bottom:1px solid #eee; vertical-align:top;\">References<\/th><td style=\"padding:10px; border-bottom:1px solid #eee; vertical-align:top;\"><p class=\"sepub-empty\">No publications found.<\/p><\/td><\/tr><\/tbody><\/table><\/div>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Restriction endonuclease EmiS6 I<\/p>\n","protected":false},"featured_media":58,"template":"","meta":[],"product_brand":[1659],"product_cat":[23],"product_tag":[1699,1700,1698,1701],"class_list":["post-5310","product","type-product","status-publish","has-post-thumbnail","product_brand-product_br_7fed80","product_cat-re","product_tag-product_ta_714772-1","product_tag-product_ta_714772-2","product_tag-product_ta_714772","product_tag-product_ta_714772-3","pa_285b89be-c12827be1b","pa_4ebaafd1-pa_4ebaafd_d66329","pa_50b54da1-pa_50b54da_6e490e","pa_be110261-fc490ca45c","pa_concentration-871595f309","pa_fasovka-65253e40ca","pa_fasovka-658e880e51","pa_obem-pa_obem_d1a3e6-7","pa_obem-pa_obem_d1a3e6-8","pa_package-d20caec3b4","pa_package-5dbc98dcc9","pa_site-pa_site_aea581-4","pa_variant-d2203cb123","first","instock","featured","taxable","shipping-taxable","product-type-variable","berocket_lgv_grid","berocket_lgv_list_grid"],"_links":{"self":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product\/5310","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media\/58"}],"wp:attachment":[{"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/media?parent=5310"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_brand?post=5310"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_cat?post=5310"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sibenzyme.com\/en\/wp-json\/wp\/v2\/product_tag?post=5310"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}