Description
Recognition site and hydrolysis position:
| CCTCGAGG | CC↑TCGAGG |
| GGAGCTCC | GGAGCT↓CC |
Source: : An E.coli strain that carries the cloned Abs I gene fromArthrobacter species 7М06
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19SE/DriI – digest in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
pUC19SE/DriI – digest
Optimal SE-buffer: SE-buffer AbsI
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 10-25 | 10-25 | 0-10 | 25-50 | 10-25 | 25 |
Storage conditions: 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 µg/ml BSA, 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme10 X SE-buffer AbsI
Methylation sensitivity: not tested
Notes: A long incubation time may result in star activity.
References:
V.A. Chernukhin, Yu.G. Kashirina, Yu.E. Tomilova, D.A. Gonchar, V.S. Dedkov, N.A. Mikhnenkova, S.Kh. Degtryarev. New Eight Bases Cutter AbsI from Arthrobacter species Recognizes Palindromic DNA Sequence 5’-CC^TCGAGG-3’ // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 2, pp 29-34, 2006
