Abs I

6,500.00 26,000.00 

  • CNY: 79.77 ¥ - 319.08 ¥

Restriction endonuclease Abs I

  • Recognition site: CC↑TCGAGG / GGAGCT↓CC
  • Источник: Из Arthrobacter species 7М06.
  • Оптимальный буфер: SE-буфер AbsI
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: E535_ Category:

Description

Recognition site:

CC↑TCGAGG
GGAGCT↓CC

Source: Arthrobacter species 7M06
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19SE/DriI -digest in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
pUC19SE/DriI – digest
Optimal SE-buffer: SE-buffer AbsI
Enzyme activity (%):

B G O W Y ROSE
75 10 0 50 0 50

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 2-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer AbsI
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: A long incubation time may result in star activity.
References:
Degtyarev S.Kh., Rechkunova N.I., Zhilkina O.A., Semenchenko G.V., Novozhilova M.I. Kurthia zopfii – a strain producer of restriction endonuclease Kzo9I // Soviet Union patent SU 1440919 (1988). (In Russian)

Available Options

SKUРackage sizePackageVariantConcentrationPrice 
E535S200 uRegular5 000 u/ml6,500.00 
E536L1000 uRegular5 000 u/ml26,000.00