Recognition site and hydrolysis position:
Source: : An E.coli strain that carries the cloned Abs I gene fromArthrobacter species 7М06
One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19SE/DriI – digest in 1 hour at 37°C in a total reaction volume of 50 μl.
pUC19SE/DriI – digest
Optimal SE-buffer: SE-buffer AbsI
Enzyme activity (%):
Storage conditions: 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 µg/ml BSA, 50% glycerol; Store at -20°C.
After 5-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme10 X SE-buffer AbsI
Methylation sensitivity: not tested
Notes: A long incubation time may result in star activity.
V.A. Chernukhin, Yu.G. Kashirina, Yu.E. Tomilova, D.A. Gonchar, V.S. Dedkov, N.A. Mikhnenkova, S.Kh. Degtryarev. New Eight Bases Cutter AbsI from Arthrobacter species Recognizes Palindromic DNA Sequence 5’-CC^TCGAGG-3’ // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 2, pp 29-34, 2006