AccB1 I

1,125.00 4,500.00 

  • CNY: 87.55 ¥ - 350.19 ¥

Restriction endonuclease AccB1 I

  • Recognition site: G↑GYRCC / CCRYG↓G
  • Источник: Acinetobacter calcoaceticus B1
  • Оптимальный буфер: SE-буфер K
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E163 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E163S500 uRegular5 000 u/ml1,125.00 
  • CNY: 87.55 ¥
E164L2500 uRegular5 000 u/ml4,500.00 
  • CNY: 350.19 ¥

Description

Recognition site and hydrolysis position:

GGYRCC G↑GYRCC
CCRYGG CCRYG↓G

Source: Acinetobacter calcoaceticus B1
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer K + BSA
Enzyme activity (%):

B G O W Y ROSE
50 10 10 75 50 30

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.
Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer K, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Degtyarev, S.Kh., Dedkov, V.S., Belichenko, O.A., Verchosina, V.A., Kusner, Y.S., Unpublished observations.