Source: Alteromonas macleodii 87
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-buffer: SE-buffer W + BSA
Enzyme activity (%):
Storage conditions: 10 mM KH2PO4 (pH 7.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Degtyarev, S.Kh., Repin, V.E., Unpublished observations.
|E017||S||1000 u||Regular||20 000 u/ml||2,300.00 ₽|
|E018||L||5000 u||Regular||20 000 u/ml||9,200.00 ₽|