Ama87 I

2,300.00 9,200.00 

  • CNY: 32.77 ¥ - 131.07 ¥

Restriction endonuclease Ama87 I

  • Recognition site: C↑YCGRG / GRGCY↓C
  • Источник: Alteromonas macleodii 87
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: E017 Category:

Description

Recognition site:

C↑YCGRG
GRGCY↓C

Source: Alteromonas macleodii 87
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W + BSA
Enzyme activity (%):

B G O W Y ROSE
10 50 75 100 0 25

Storage conditions: 10 mM KH2PO4 (pH 7.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Degtyarev, S.Kh., Repin, V.E., Unpublished observations.

Available Options

SKUРackage sizePackageVariantConcentrationPrice 
E017S1000 uRegular20 000 u/ml2,300.00 
E018L5000 uRegular20 000 u/ml9,200.00