AsuNH I

6,000.00 24,000.00 

  • CNY: 467.94 ¥ - 1,871.76 ¥

Restriction endonuclease AsuNH I

  • Recognition site: G↑CTAGC / CGATC↓G
  • Источник: Из Actinobacillus suis NH
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E063 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E063S1000 uRegular20 000 u/ml6,000.00 
  • CNY: 467.94 ¥
E064L5000 uRegular20 000 u/ml24,000.00 
  • CNY: 1,871.76 ¥
E064TL5000 uTurbo20 000 u/ml24,000.00 
  • CNY: 1,871.76 ¥
E063TS1000 uTurbo20 000 u/ml6,000.00 
  • CNY: 467.94 ¥

Description

Recognition site and hydrolysis position:

GCTAGC G↑CTAGC
CGATCG CGATC↓G

Source: Actinobacillus suis NH
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (HindIII-digest)
Optimal SE-buffer: SE-buffer Y + BSA
Enzyme activity (%):

B G O W Y ROSE
75 50 0 0 100 25

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Dedkov, V.S., Degtyarev, S.Kh. Biol. Chem. 379: 573-574 (1998).