Description
Recognition site and hydrolysis position:
| ACTGGN | ACTGGN↑ |
| TGACCN | TGAC↓CN |
Source: Bacillus stearothermophilus 1
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 75 | 75 | 25 | 10 | 100 | 100 |
Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme >95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 65°C.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Repin, V.E., Degtyarev, S.Kh. Unpublished observations.
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