DNA polymerase I E.coli (Klenow Fragment)

1,280.00 5,120.00 

  • CNY: 18.24 ¥ - 72.94 ¥

  • Источник: Выделен из штамма E.coli, содержащего ген ДНК-полимеразы (Фрагмент Кленова)
  • Оптимальный буфер: SE-буфер Фрагмент Кленова
  • Оптимальная температура: 37
SKU: E325 Category:

Description

DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3` -> 5` exonuclease activity, but has lost 5` -> 3` exonuclease activity.
Applications:
– dideoxy sequencing;
– polishing ends;
– second strand cDNA synthesis

Source: Isolated from E.coli strain that carries the cloned DNA Polymerase I, Large (Klenow) Fragment gene.

Unit definition: DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3` -> 5` exonuclease activity, but has lost 5` -> 3` exonuclease activity.
Applications:
– dideoxy sequencing;
– polishing ends;
– second strand cDNA synthesis

Reaction buffer: SE-buffer Klenow fragment

Storage conditions: 10 mM KH2PO4 (pH 7.5); 50 mM KCl; 0.5 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.

Quality control: The enzyme is purified free of contaminating endonucleases andexonucleases.

Available Options

SKUРackage sizePackageConcentrationPrice 
E325S200 u5 000 u/ml1,280.00 
E326L1000 u5 000 u/ml5,120.00