Pcs I

5,500.00 22,000.00 

  • CNY: 78.36 ¥ - 313.43 ¥

MD DNA endonuclease Pcs I.
The enzyme cleaves C5-methylated DNA and doesn’t cut unmodified DNA.

  • Концентрация: 1000 е.а./мл
  • Recognition site: (5mC)GNNNNN↑NN(5mC)G / G(5mC)NN↓NNNNNG(5mC)
  • Источник: Paracoccus carotinifaciens 3K
  • Оптимальный буфер: SE-буфер PcsI
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: E505 Category:


Recognition site:


Source: Paracoccus carotinifaciens 3K

Substrate specificity:

Unit definition: One unit is defined as the amount of enzyme required to digest a unique site
in 1 μg of DNA pMHgaI/DriI in 1 hour at 37°C in a total reaction volume of 50 μl.

PcsI activity assay on DNA pMHgaI/DriI

1 and 6 – 1 Kb SE DNA-markers
2 – Control pMHgaI/DriI
3 – 0.5 μl Pcs I
4 – 1 μl Pcs I
5 – 2 μl Pcs I

Products were separated in 1,4% agarose gel in Buffer TAE.

PcsI activity assay on DNA pMHgaI/DriI

Assayed on: pMHgaI/DriI is a linearized plasmid pMHgaI, which carries genes of DNA-methyltransferases M1.HgaI (recognition sequence 5`-GCGTC-3`) and M2.HgaI (5`-GACGC-3`) and includes a unique PcsI canonical site:
3`-WG(5mC)NNNNNNNG(5mC)W-5` [1].
Optimal SE-buffer: SE-buffer PcsI
Enzyme activity (%):

50 25 0 10 50 20

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 0.1 mg/ml BSA, 50% glycerol; Store at -20°C.

Ligation: –

Non-specific hydrolisis: No detectable degradation of 1μg of Lambda DNA was observed after incubation with 1 units of enzyme for 16 hours at 37°C in a total reaction volume of 50 μl.Reagents Supplied with Enzyme

10 X SE-buffer PcsI

Methylation sensitivity: The enzyme cleaves only C5-methylated DNA and does not cut unmodified DNA [1].

Notes: When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion.


1. Chernukhin V.A., Nayakshina T.N., Tarasova M.V., Golikova L.N., Akishev A.G., Dedkov V.S., Mikhnenkova N.A., Degtyarev S.Kh. Bacterial strain Paracoccus carotinifaciens 3K- producer of PcsI site specific endonuclease. // Russian Federation patent RU 2377294 C1 (2009).


Available Options

SKUРackage sizePackagePrice 
E505S50 u5,500.00 
E506L250 u22,000.00