PspC I

2,800.00 11,200.00 

  • CNY: 24.57 ¥ - 98.27 ¥

Restriction endonuclease PspC I

  • Recognition site: CAC↑GTG / GTG↓CAC
  • Источник: Pseudomonas species C
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E475 Category:

Description

Recognition site and hydrolysis position:

CACGTG CAC↑GTG
GTGCAC GTG↓CAC

Source: Pseudomonas species C
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 50 0 0 50 5

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol.
Storage temperature: -20°C.
Storage at -70°C is recommended for periods longer than 30 days.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Ligation is better in presence of 10% PEG.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Tronin, A., Kileva, E.V., Mezentseva, N., Dedkov, V.S., Degtyarev, S.K. Unpublished observations. (2003)

Available Options

SKUРackage sizePackageVariantConcentrationPrice 
E475TS2000 uTurbo20 000 u/ml2,800.00 
E476TL10000 uTurbo20 000 u/ml11,200.00 
E475S2000 uRegular20 000 u/ml2,800.00 
E476L10000 uRegular20 000 u/ml11,200.00