Tru9 I

3,300.00 ₽ – 13,200.00 ₽

  • CNY: 257.70 ¥ - 1,030.79 ¥

Restriction endonuclease Tru9 I

  • Recognition site: T↑TAA / AAT↓T
  • Источник: Из штамма E.coli несущего клонированный ген Tru9I из Thermus ruber 9
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 65
  • Температура инактивации, 20 мин при: 80
SKU: SE-E199 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E199TS500 uTurbo20 000 u/ml3,300.00 ₽
  • CNY: 257.70 ¥
E200TL2500 uTurbo20 000 u/ml13,200.00 ₽
  • CNY: 1,030.79 ¥
E199S500 uRegular20 000 u/ml3,300.00 ₽
  • CNY: 257.70 ¥
E200L2500 uRegular20 000 u/ml13,200.00 ₽
  • CNY: 1,030.79 ¥

Description

Recognition site and hydrolysis position:

TTAA T↑TAA
AATT AAT↓T

Source: An E.coli strain that carries the cloned Tru9 I gene from Thermus ruber 9
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

B G O W Y ROSE
75 25 25 100 50 100

Storage conditions: 10 mM Tris-HCl-(pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 65°C. Reagents Supplied with Enzyme 10 X SE-buffer W
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by TTAmA methylation .
Notes:
References:
Prichodko, E.A., Rechkunova, N.I., Degtyarev, S.Kh. Sib. Biol. J. 1:57-59 (1991).
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006

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