Fal I

Description

Recognition site and hydrolysis position:

Source: Flavobacterium aquatile Ob10
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme 20% of the DNA fragments can be ligated and of these 80% can be recut. In the presence of 10%PEG ligation is better.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W, SAM
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration results in star activity
To obtain 100% activity, SAM should be added to a final concentration 0.01 mM
References:
Dedkov, V.S., Sinichkina, S.A., Abdurashitov, M.A., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).