Description
T7 RNA Polymerase catalyzes the synthesis of RNA in the 5` -> 3` direction in the presence of a DNA template containing a T7 phage promoter.
Applications:
-Radiolabeled RNA probe preparation
-RNA generation for in vitro translation
-RNA generation for studies of RNA structure,processing and catalysis
Source: Isolated from E.coli strain that carries the cloned T7 gene I
Unit definition: T7 RNA Polymerase catalyzes the synthesis of RNA in the 5` -> 3` direction in the presence of a DNA template containing a T7 phage promoter.
Applications:
-Radiolabeled RNA probe preparation
-RNA generation for in vitro translation
-RNA generation for studies of RNA structure,processing and catalysis
Reaction buffer: SE-buffer RNA polymerase T7
Storage conditions: 50 mM Tris – HCl (pH 7.5); 100 mM NaCl; 20 mM 2-mercaptoethanol; 1 mM EDTA; 0.1% Triton X-100; 50% glycerol. Store at -20°C.
Quality control: The enzyme is purified free of contaminating endonucleases andexonucleases.
