Acc65 I

Acc65 I

Price range: 3 400 ₽ through 13 600 ₽

Restriction endonuclease Acc65 I

  • Recognition site:
    5'… G▼GTACC …3'
    3'… CCATG▲G …5'
  • Source: Acinetobacter calcoaceticus 65
  • Optimal buffer: SE-buffer W
  • Optimal temperature: 37 °C
  • Inactivated during 20 minutes at: 65 °C
SKU: SE-E003 Category:

SKUРackagePackageVariantConcentrationPrice 
E003S1000 uRegular20 000 u/ml3 400 ₽
E004L5000 uRegular20 000 u/ml13 600 ₽
E003TS1000 uTurbo20 000 u/ml3 400 ₽
E004TL5000 uTurbo20 000 u/ml13 600 ₽

Description

Recognition site and hydrolysis position:

GGTACC G↑GTACC
CCATGG CCATG↓G

Source: Acinetobacter calcoaceticus 65
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA(dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA(dcm-)
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

B G O W Y ROSE
10 25 75 100 10 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by overlapping Dcm methylation(CmCWGG): GGTACCWGG.
Notes:
References:
Prichodko, G.G., Rechkunova, N.I., Repin, V.E., Degtyarev, S.Kh., Sib. Biol. J. 1: 59-60 (1991).

Additional Information

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