AclW I

2,300.00 ₽ – 9,200.00 ₽

  • CNY: 187.04 ¥ - 748.14 ¥

Restriction endonuclease AclW I

  • Recognition site: GGATC(N)4↑ / CCTAG(N)5↓
  • Источник: Acinetobacter calcoaceticus W2131
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E211 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E211S100 uRegular1 000-3 000 u/ml2,300.00 ₽
  • CNY: 187.04 ¥
E212L500 uRegular1 000-3 000 u/ml9,200.00 ₽
  • CNY: 748.14 ¥

Description

Recognition site and hydrolysis position:

GGATC(N)4 GGATC(N)4↑
CCTAG(N)5 CCTAG(N)5↓

Source: Acinetobacter calcoaceticus W2131
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (dam-)
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 50 0 0 100 30

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated with T4 DNA ligase and recut. In the presence of 10% PEG ligation is better.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 6 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by Dam methylation (GmATC) GGATCNotes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Abdurashitov, M.A., Belichenko, O.A., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations. (1994)

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