Ars I

2,875.00 ₽ – 11,500.00 ₽

  • CNY: 233.80 ¥ - 935.18 ¥

Restriction endonuclease Ars I

  • Recognition site: ↑(N)8GACNNNNNNTTYG(N)11↑ / ↓(N)13CTGNNNNNNAARC(N)6↓
  • Источник: Arthrobacter species NTS
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 30
  • Температура инактивации, 20 мин при: 65
SKU: SE-E575 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E575S50 uRegular500 u/ml2,875.00 ₽
  • CNY: 233.80 ¥
E576L250 uRegular500 u/ml11,500.00 ₽
  • CNY: 935.18 ¥

Description

Recognition site and hydrolysis position:

(N)8GACNNNNNNTTYG(N)11 ↑(N)8GACNNNNNNTTYG(N)11↑
(N)13CTGNNNNNNAARC(N)6 ↓(N)13CTGNNNNNNAARC(N)6↓

Source: Arthrobacter species NTS
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 30°C in a total reaction volume of 50 μl.
Assayed on:
T7 DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
0 0 0 0 100 20

Storage conditions: 10 mM KH2PO4(pH 7.4); 200 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme 70% of DNA fragments can be ligated. Of these 80% can be recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 1 u.a. of enzyme for 16 hours at 30°C. Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:

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