Bpm I

3,220.00 ₽ – 12,880.00 ₽

  • CNY: 261.85 ¥ - 1,047.40 ¥

Restriction endonuclease Bpm I

  • Recognition site: CTGGAG(N)16↑ / GACCTC(N)14↓
  • Источник: Bacillus pumilus
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E467 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E467S50 uRegular500-1 000 u/ml3,220.00 ₽
  • CNY: 261.85 ¥
E468L250 uRegular500-1 000 u/ml12,880.00 ₽
  • CNY: 1,047.40 ¥

Description

Recognition site and hydrolysis position:

CTGGAG(N)16 CTGGAG(N)16↑
GACCTC(N)14 GACCTC(N)14↓

Source: Bacillus pumilus
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W + BSA
Enzyme activity (%):

B G O W Y ROSE
25 50 75 100 50 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C
Ligations:
After 2-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and 95% may be recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by overlapping Dcm-methylation (CmCWGG):
5′- C(5mC)TGGAG(N)16-3′
3′- GGA(5mC)CTC(N)14-5′
Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Degtyarev, S.Kh., Morgan P. Unpublished observation (1992).

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