BssNA I

6,000.00 ₽ – 24,000.00 ₽

  • CNY: 487.92 ¥ - 1,951.68 ¥

Restriction endonuclease BssNA I

  • Recognition site: GTA↑TAC / CAT↓ATG
  • Источник: Bacillus stearothermophilus NA
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: N
SKU: SE-E261 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E261S1000 uRegular10 000 u/ml6,000.00 ₽
  • CNY: 487.92 ¥
E262L5000 uRegular10 000 u/ml24,000.00 ₽
  • CNY: 1,951.68 ¥

Description

Recognition site and hydrolysis position:

GTATAC GTA↑TAC
CATATG CAT↓ATG

Source: Bacillus stearothermophilus NA
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W + BSA
Enzyme activity (%):

B G O W Y ROSE
50 50 75 100 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Shinkarenko, N.M., Dedkov, V.S., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1996).

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