BstV1 I

4,370.00 ₽ – 17,480.00 ₽

  • CNY: 355.37 ¥ - 1,421.47 ¥

Restriction endonuclease BstV1 I

  • Recognition site: GCAGC(N)8↑ / CGTCG(N)12↓
  • Источник: Bacillus stearothermophilus V1
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 80
SKU: SE-E303 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E303S100 uRegular1 000-2 000 u/ml4,370.00 ₽
  • CNY: 355.37 ¥
E304L500 uRegular1 000-2 000 u/ml17,480.00 ₽
  • CNY: 1,421.47 ¥

Description

Recognition site and hydrolysis position:

GCAGC(N)8 GCAGC(N)8↑
CGTCG(N)12 CGTCG(N)12↓

Source: Bacillus stearothermophilus V1
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
pBR322 DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
75 100 75 75 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer G
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: At 37°C activity is 10% from maximum.
References:
Dedkov, V.S., Muradov, S.V., Shinkarenko, N.M., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).

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