Description
Recognition site and hydrolysis position:
| GCAGC(N)8 | GCAGC(N)8↑ |
| CGTCG(N)12 | CGTCG(N)12↓ |
Source: Bacillus stearothermophilus V1
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
pBR322 DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 75 | 100 | 75 | 75 | 75 | 100 |
Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 55°C.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: At 37°C activity is 10% from maximum.
References:
Dedkov, V.S., Muradov, S.V., Shinkarenko, N.M., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).
Additional Information
Related products
-
Acs I
Price range: 4 100 ₽ through 16 400 ₽ Select options This product has multiple variants. The options may be chosen on the product page -
Ahl I
Price range: 5 300 ₽ through 21 200 ₽ Select options This product has multiple variants. The options may be chosen on the product page -
AccBS I
Price range: 5 800 ₽ through 23 200 ₽ Select options This product has multiple variants. The options may be chosen on the product page
Войти/зарегистр.