Description
Description: Exonuclease III (E. coli) catalyzes the stepwise removal of mononucleotides from 3`- hydroxyl termini of double stranded DNA. Exonuclease III activity depends partially on helical structure and displays sequence dependence (C > A = T > G). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications.
Applications:
– unidirectional nested deletions;
– site-directed mutagenesis;
– preparation of strand-specific probes;
– preparation of single-stranded substrates for dideoxy sequencing.
Source: Isolated from E.coli.
Unit definition: Description: Exonuclease III (E. coli) catalyzes the stepwise removal of mononucleotides from 3`- hydroxyl termini of double stranded DNA. Exonuclease III activity depends partially on helical structure and displays sequence dependence (C > A = T > G). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications.
Applications:
– unidirectional nested deletions;
– site-directed mutagenesis;
– preparation of strand-specific probes;
– preparation of single-stranded substrates for dideoxy sequencing.
Reaction buffer: SE-buffer Exonuclease III
Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.5 mM EDTA; 1 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Quality control:
Notes:
