Description
Recognition Site and Hydrolysis Position:
Source: Exiguobacterium mexicanum 6
Definition of Activity Unit: One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Substrate for Activity Determination: λ DNA
Optimal Buffer: SE buffer Y
Enzyme Activity in Various Buffers as a Percentage of Maximum:
| B | G | O | W | Y | ROSE |
| 75-100 | 50-75 | 25-50 | 25-50 | 100 | 25 |
Storage Conditions: 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 µg/ml BSA, 50% glycerol; Store at -20°C
Ligase Compatibility: After 5-fold overdigestion with Emi I, 95% of the DNA fragments can be ligated with T4 DNA Ligase and 80% of those can be recut
Non-Specific Hydrolysis: A 50 µl reaction containing 1 µg of DNA and 10 units of enzyme incubated for 16 hours
resulted in the same pattern of DNA bands as a reaction incubated for 1 hour.
Oligonucleotide Assay: No detectable degradation of a single-stranded and double-stranded oligonucleotide was observed after incubation with 5 units of restriction endonuclease for 3 hours.
Supplied with the Enzyme: 10x SE Buffer Y
Enzyme Properties: When using a buffer other than the optimal (supplied) SE-Buffer, it may be necessary to add more enzyme to achieve complete digestion.
Reference: Abdurashitov M.A., Gonchar D.A., Chernukhin V.A., Dedkov V.S., Mikhnenkova N.A., Nikonova A.A., Degtyarev S.Kh. (2025) The restriction endonuclease EmiI, an isoschizomer of Ksp632I, recognizes the non-palindromic DNA sequence 5′-CTCTTC(1/4)-3′. DNA-Processing Enzymes, 2025, Vol. 4.
