FriO I

2,875.00 ₽ – 11,500.00 ₽

  • CNY: 233.80 ¥ - 935.18 ¥

Restriction endonuclease FriO I

  • Recognition site: GRGCY↑C / C↓YCGRG
  • Источник: Flavobacterium rigense O
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E157 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E157S1000 uRegular20 000 u/ml2,875.00 ₽
  • CNY: 233.80 ¥
E158L5000 uRegular20 000 u/ml11,500.00 ₽
  • CNY: 935.18 ¥

Description

Recognition site and hydrolysis position:

GRGCYC GRGCY↑C
CYCGRG C↓YCGRG

Source: Flavobacterium rigense O
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 75 10 0 100 25

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Belichenko, O.A., Prikhod’ko, E.A., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1994).

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