Description
Hot Start Taq DNA Polymerase is complex mixture of a thermostable 94 kD Taq DNA Polymerase purified from E.coli recombinant strain expressing Thermus aquaticus polymerase gene and specific monoclonal antibodies from mouse.Hot StartTaq DNA Polymerase is inactive under conditions of amplification reaction preparation. It can eliminate amplification artefacts such as primer-dimer formation and mispriming during preamplification stage and thus may provide improved specificity when compared to standard DNA polymerases. An advantage of Hot Start Taq DNA Polymerase is the absence of additional heating step for polymerase activation. Heat activation of enzyme occurs during the first denaturation step. An inactive complex of Hot Start Taq DNA Polymerase dissociates automatically over + 70°C, allowing activation of DNA polymerase.
Applications:
– Highly specific PCR;
– Multiplex PCR (highly recommended);
– High sensitivity applications.
Source: Isolated from E.coli strain that carries the cloned Taq DNA polymerase gene from Thermus aquaticus YT1
Unit definition: Hot Start Taq DNA Polymerase is complex mixture of a thermostable 94 kD Taq DNA Polymerase purified from E.coli recombinant strain expressing Thermus aquaticus polymerase gene and specific monoclonal antibodies from mouse.Hot StartTaq DNA Polymerase is inactive under conditions of amplification reaction preparation. It can eliminate amplification artefacts such as primer-dimer formation and mispriming during preamplification stage and thus may provide improved specificity when compared to standard DNA polymerases. An advantage of Hot Start Taq DNA Polymerase is the absence of additional heating step for polymerase activation. Heat activation of enzyme occurs during the first denaturation step. An inactive complex of Hot Start Taq DNA Polymerase dissociates automatically over + 70°C, allowing activation of DNA polymerase.
Applications:
– Highly specific PCR;
– Multiplex PCR (highly recommended);
– High sensitivity applications.
Reaction buffer: SE-buffer Hot Start Taq DNA polymerase
Storage conditions: 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50 % glycerol, 0.5 % Nonidet P-40, 0.5 % Tween-20. Store at -20°C.
Notes: The recommended amount of enzyme is 1 u per 50μl of a total reaction volume.
Legal Licenses/Patents/Disclaimers:
Some applications in which this product can be used may be covered by patents issued and applicable in the United States, European Union and certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.
