Description
Recognition site and hydrolysis position:
| G(mA)TC | G(mA)↑TC |
| CT(mA)G | CT↓(mA)G |
Source: Marinococus albus I
Substrate specificity:
Unit definition: One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA (dam-methylated) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on: pBR322 DNA (dam-methylated)
Optimal SE-buffer: SE-buffer MalI
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 25 | 25 | 50 | 75 | 50 | 50 |
Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol; Store at -20°C.
Ligation: After 5-fold overdigestion with enzyme more than 20% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.Reagents Supplied with Enzyme
10 X SE-buffer MalI
Methylation sensitivity: The enzyme cleaves only methylated DNA.
Notes: References:
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