Mbo II

2,970.00 ₽ – 11,880.00 ₽

  • CNY: 241.52 ¥ - 966.08 ¥

Restriction endonuclease Mbo II

  • Recognition site: GAAGA(N)8↑ / CTTCT(N)7↓
  • Источник: Из штамма E.coli несущего клонированный ген Mbo II из Moraxella bovis
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E471 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E471S200 uRegular5 000 u/ml2,970.00 ₽
  • CNY: 241.52 ¥
E472L1000 uRegular5 000 u/ml11,880.00 ₽
  • CNY: 966.08 ¥

Description

Recognition site and hydrolysis position:

GAAGA(N)8 GAAGA(N)8↑
CTTCT(N)7 CTTCT(N)7↓

Source: An E.coli strain, that carries the cloned gene Mbo II from Moraxella bovis
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (dam-)
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 75 25 50 100 50

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme approximately 60% of the DNA fragments can be ligated and recut. In presence of 10%PEG ligation is better.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by overlappin dam-methylation(GmATC): GAAGATC.
Notes:
References:
Brown N.L., Hutchison C.A. III, Smith M. J. Mol. Biol. 140: 143-148 (1980).

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