Mfe I

4,070.00 ₽ – 16,280.00 ₽

  • CNY: 330.97 ¥ - 1,323.89 ¥

Restriction endonuclease Mfe I

  • Recognition site: C↑AATTG / GTTAA↓C
  • Источник: Из штамма E.coli несущего клонированный ген Mfe I из Mycoplasma fermentans.
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: N
SKU: SE-E295 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E295TS1000 uTurbo20 000 u/ml4,070.00 ₽
  • CNY: 330.97 ¥
E296TL5000 uTurbo20 000 u/ml16,280.00 ₽
  • CNY: 1,323.89 ¥
E295S1000 uRegular20 000 u/ml4,070.00 ₽
  • CNY: 330.97 ¥
E296L5000 uRegular20 000 u/ml16,280.00 ₽
  • CNY: 1,323.89 ¥

Description

Recognition site and hydrolysis position:

CAATTG C↑AATTG
GTTAAC GTTAA↓C

Source: An E.coli strain, that carries the cloned gene Mfe I from Mycoplasma fermentans.
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 75 10 25 75 25

Storage conditions: 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity:
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:

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