Mnl I

5,060.00 ₽ – 20,240.00 ₽

  • CNY: 411.48 ¥ - 1,645.92 ¥

Restriction endonuclease Mnl I

  • Recognition site: CCTC(N)7↑ / GGAG(N)6↓
  • Источник: Из штамма E.coli несущего клонированный ген Mnl I из Moraxella nonliquefaciens
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E481 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E481TS500 uTurbo10 000 u/ml5,060.00 ₽
  • CNY: 411.48 ¥
E482TL2500 uTurbo10 000 u/ml20,240.00 ₽
  • CNY: 1,645.92 ¥
E481S500 uRegular10 000 u/ml5,060.00 ₽
  • CNY: 411.48 ¥
E482L2500 uRegular10 000 u/ml20,240.00 ₽
  • CNY: 1,645.92 ¥

Description

Recognition site and hydrolysis position:

CCTC(N)7 CCTC(N)7↑
GGAG(N)6 GGAG(N)6↓

Source: An E.coli strain, that carries the cloned gene Mnl I from Moraxella nonliquefaciens
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
75 100 25 25 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme 50% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by overlapping CG methylation: CCTmCG.
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Brinkley, P., Bautista, D.S., Graham, F.L.; Gene 100: 267-268 (1991).

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