PciS I

PciS I

Price range: 6 200 ₽ through 24 800 ₽

Restriction endonuclease PciS I

  • Recognition site:
    5'… GCTCTTCN▼ …3'
    3'… CGAGAAG(N)4▲ …5'
  • Source: Planococcus citreus S
  • Optimal buffer: SE-buffer B
  • Optimal temperature: 37 °C
  • Inactivated during 20 minutes at: 65 °C
SKU: SE-E497 Category:

SKUРackagePackageVariantConcentrationPrice 
E497S50 uRegular500-2 000 u/ml6 200 ₽
E498L250 uRegular500-2 000 u/ml24 800 ₽

Description

Recognition site and hydrolysis position:

GCTCTTCN GCTCTTCN↑
CGAGAAG(N)4 CGAGAAG(N)4↓

Source: Planococcus citreus S
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 50 0 0 75 50

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA and 50% glycerol; Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and 95% of these can be recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 0.5 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Chernukhin, V.A., Kileva, E.V., Tomilova, J.E., Dedkov, V.S. Unpublished observations (2006).

Additional Information

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