Description
Recognition site and hydrolysis position:
| GCTCTTCN | GCTCTTCN↑ |
| CGAGAAG(N)4 | CGAGAAG(N)4↓ |
Source: Planococcus citreus S
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 100 | 50 | 0 | 0 | 75 | 50 |
Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA and 50% glycerol; Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and 95% of these can be recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 0.5 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Chernukhin, V.A., Kileva, E.V., Tomilova, J.E., Dedkov, V.S. Unpublished observations (2006).
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