Rig I

7,500.00 ₽ – 30,000.00 ₽

  • CNY: 609.90 ¥ - 2,439.60 ¥

Restriction endonuclease Rig I

  • Recognition site: GGCCGG↑CC / CC↓GGCCGG
  • Источник: Rhizobium yangligense
  • Оптимальный буфер: SE-буфер RigI
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E529 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E529S100 uRegular2 000 u/ml7,500.00 ₽
  • CNY: 609.90 ¥
E530L500 uRegular2 000 u/ml30,000.00 ₽
  • CNY: 2,439.60 ¥

Description

Recognition site and hydrolysis position:

GGCCGGCC GGCCGG↑CC
CCGGCCGG CC↓GGCCGG

Source: Rhizobium yangligense
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Ad2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Adenovirus -2 DNA
Optimal SE-buffer: SE-buffer RigI
Enzyme activity (%):

B G O W Y ROSE
75 50 0 10 50 10

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.
Storage at -20°C.
Storage at -70°C is recommended for periods longer than 7 days.
Ligations:
After 3-fold overdigestion with enzyme > 95% of Ad2 DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 4 units of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer RigI, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by mCG or GmC methylation:
5`-GGC(m5C)GGCC-3`/3`-CCGG(m5C)CGG-5` or
5`-GG(m5C)CGG(m5C)C-3`/3`-C(m5C)GGC(m5C)GG-5`
Notes: Do not use BSA for long incubation.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
References:

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