Sbf I

3,450.00 ₽ – 13,800.00 ₽

  • CNY: 280.55 ¥ - 1,122.22 ¥

Restriction endonuclease Sbf I

  • Recognition site: CCTGCA↑GG / GG↓ACGTCC
  • Источник: Из штамма E.coli несущего клонированный ген Sbf I из Streptomyces species Bf61
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 80
SKU: SE-E101 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E101S200 uRegular5 000 u/ml3,450.00 ₽
  • CNY: 280.55 ¥
E102L1000 uRegular5 000 u/ml13,800.00 ₽
  • CNY: 1,122.22 ¥

Description

Recognition site and hydrolysis position:

CCTGCAGG CCTGCA↑GG
GGACGTCC GG↓ACGTCC

Source: An E.coli strain that carries the cloned Sbf I gene from Streptomyces species Bf61
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 50 0 0 100 50

Storage conditions: 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol ; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and of these more than 90% canrecut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity.
References:
Abdurashitov, M.A., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations (1994).

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