Sfr274 I

1,870.00 ₽ – 7,480.00 ₽

  • CNY: 152.07 ¥ - 608.27 ¥

Restriction endonuclease Sfr274 I

  • Recognition site: C↑TCGAG / GAGCT↓C
  • Источник: Streptomyces fradiae 274
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 50
  • Температура инактивации, 20 мин при: 65
SKU: SE-E125 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E125S2000 uRegular20 000 u/ml1,870.00 ₽
  • CNY: 152.07 ¥
E126L10000 uRegular20 000 u/ml7,480.00 ₽
  • CNY: 608.27 ¥
E125TS2000 uTurbo20 000 u/ml1,870.00 ₽
  • CNY: 152.07 ¥
E126TL10000 uTurbo20 000 u/ml7,480.00 ₽
  • CNY: 608.27 ¥

Description

Recognition site and hydrolysis position:

CTCGAG C↑TCGAG
GAGCTC GAGCT↓C

Source: Streptomyces fradiae 274
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (Hind III-digest) in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (Hind III-digest)
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 75 50 50 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.. Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 50°C. Reagents Supplied with Enzyme 10 X SE-buffer B.
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by CTCGmAG methylation.
Not blocked by CTmCGAG methylation.
Notes: Incubation at 37°C results in 70% activity
References:
Puchkova, L.I., Krivopalova, G.N., Andreeva, I.S., Selina, A.V., Serov, G.D., Rechkunova, N.I., Degtyarev, S.Kh. Izv. Sib. Otd. Akad. Nauk SSSR 1: 32-34 (1990).

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