Description
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5` -> 3` direction and requires the presence of template and primer. This enzyme has a 3` -> 5` exonuclease activity.
Applications:
– polishing ends;
– probe labeling using replacement synthesis.
Source: Isolated from E.coli strain that carries the cloned T4 DNA polymerase gene
Unit definition: T4 DNA Polymerase catalyzes the synthesis of DNA in the 5` -> 3` direction and requires the presence of template and primer. This enzyme has a 3` -> 5` exonuclease activity.
Applications:
– polishing ends;
– probe labeling using replacement synthesis.
Reaction buffer: SE-buffer DNA polymerase T4
Storage conditions: 20 mM Tris-HCl (pH 7.5); 50 mM KCl; 10 mM 2-mercaptoethanol ; 50% glycerol. Store at -20°C.
Quality control: The enzyme is purified free of contaminating endonucleases andexonucleases.
