Description
Taq DNA polymerase is a thermostable DNA polymerase that possesses a 5` -> 3` polymerase activity and a double strand specific 5` -> 3` exonuclease activity.
Applications:
– nick translation;
– primer extension reaction.
Source: Isolated from E.coli strain that carries the cloned Taq DNA polymerase gene from Thermus aquaticus.
Unit definition: Taq DNA polymerase is a thermostable DNA polymerase that possesses a 5` -> 3` polymerase activity and a double strand specific 5` -> 3` exonuclease activity.
Applications:
– nick translation;
– primer extension reaction.
Reaction buffer: SE-buffer DNA polymerase Taq (Mg2+ free)
Storage conditions: 20 mM Tris-HCl (pH 7.6); 100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5% Triton X-100; 50% glycerol. Store at -20°C.
Quality control: E.coli DNA free.
The enzyme is purified free of contaminating endonucleases andexonucleases.
Legal Licenses/Patents/Disclaimers:
Some applications in which this product can be used may be covered by patents issued and applicable in the United States, European Union and certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.
