Source: Acinetobacter calcoaceticus 16
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):
Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
After 5-fold overdigestion with enzyme about >80% of the DNA fragments can be ligated and recut.
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Degtyarev, S.Kh., Repin, V.E. unpublished observations. 1992
|E001||S||200 u||Regular||5 000 u/ml||1,400.00 ₽|
|E002||L||1000 u||Regular||5 000 u/ml||5,600.00 ₽|