Acc16 I

1,400.00 5,600.00 

  • CNY: 19.85 ¥ - 79.39 ¥

Restriction endonuclease Acc16 I

  • Recognition site: TGC↑GCA / ACG↓CGT
  • Источник: Acinetobacter calcoaceticus 16
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: E001 Category:

Description

Recognition site:

TGC↑GCA
ACG↓CGT

Source: Acinetobacter calcoaceticus 16
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

B G O W Y ROSE
50 75 25 100 75 70

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme about >80% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Degtyarev, S.Kh., Repin, V.E. unpublished observations. 1992

Available Options

SKUРackage sizePackageVariantConcentrationPrice 
E001S200 uRegular5 000 u/ml1,400.00 
E002L1000 uRegular5 000 u/ml5,600.00