Zrm I

2,970.00 11,880.00 

  • CNY: 232.02 ¥ - 928.07 ¥

Restriction endonuclease Zrm I

  • Recognition site: AGT↑ACT / TCA↓TGA
  • Источник: Zoogloea ramigera SCA
  • Оптимальный буфер: SE-буфер Y + BSA
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E005 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E005TS1000 uTurbo20 000 u/ml2,970.00 
  • CNY: 232.02 ¥
E006TL5000 uTurbo20 000 u/ml11,880.00 
  • CNY: 928.07 ¥
E005S1000 uRegular20 000 u/ml2,970.00 
  • CNY: 232.02 ¥
E006L5000 uRegular20 000 u/ml11,880.00 
  • CNY: 928.07 ¥

Description

Recognition site and hydrolysis position:

AGTACT AGT↑ACT
TCATGA TCA↓TGA

Source: Zoogloea ramigera SCA
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
50 25 0 0 100 50

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 70% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Nadeev, A.N., Kileva, E.V., Popichenko, D.V., Dedkov, V.S., Degtyarev, S.K. Unpublished observations.(2003)