Source: Acinetobacter calcoaceticus B7
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Lambda DNA (dcm-)
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):
Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
After 5-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by overlapping Dcm methylation (CmCWGG): CCANNNCCTGG or CCAGGNNNTGG.
Notes: High enzyme concentration may result in star activity.
Dedkov, V.S., Repin, V.E., Rechkunova, N.I., Degtyarev, S.Kh., Verhosina, V.A.,Vinogradova, T.P, Izv. Sib. Otd. Akad. Nauk SSSR 1: 35-37 (1990).
|E179||S||200 u||Regular||5 000 u/ml||940.00 ₽|
|E180||L||1000 u||Regular||5 000 u/ml||3,760.00 ₽|