Source: Acinetobacter calcoaceticus
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA(dam-, dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):
Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
After 3-fold overdigestion with AcoI more than 90% of Lambda DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of AcoI for 16 hours at 37°C.
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by overlapping dcm methylation (CmCWGG): CCTGGCCR.
Chernukhin, V.A., Belichenko, O.A., Tomilova, J.E., Dedkov, V.S., Doroganov, A.O.. Unpublished observations. (2006)
|E500||L||500 u||Regular||500-2 000 u/ml||11,200.00 ₽|
|E499||S||100 u||Regular||500-2 000 u/ml||2,800.00 ₽|