BstV2 I

Description

Recognition site:

Source: Bacillus stearothermophilus V2
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Sinichkina, S.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (2000).
Dedkov V.S., Degtyarev S.Kh., Rechkunova N.I., Serov G.D., Puchkova L.I., Vaitkiavichus D.P., Kyudulene E.-L.Yu., Janulaitis E.-A.A. Staphilococcus saprophyticus – a strain producer of restriction endonuclease SsrI.// Soviet Union patent SU 1486512 (1989). (In Russian)