BstV2 I

3,300.00 13,200.00 

  • CNY: 257.37 ¥ - 1,029.47 ¥

Restriction endonuclease BstV2 I

  • Recognition site: GAAGAC(N)2↑ / CTTCTG(N)6↓
  • Источник: Bacillus stearothermophilus V2
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 65
SKU: SE-E297 Category:

Available Options

E297TS200 uTurbo5 000 u/ml3,300.00 
  • CNY: 257.37 ¥
E298TL1000 uTurbo5 000 u/ml13,200.00 
  • CNY: 1,029.47 ¥
E297S200 uRegular5 000 u/ml3,300.00 
  • CNY: 257.37 ¥
E298L1000 uRegular5 000 u/ml13,200.00 
  • CNY: 1,029.47 ¥


Recognition site and hydrolysis position:


Source: Bacillus stearothermophilus V2
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

75 75 25 25 100 70

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Sinichkina, S.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (2000).
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006