BstV2 I

3,300.00 13,200.00 

  • CNY: 257.37 ¥ - 1,029.47 ¥

Restriction endonuclease BstV2 I

  • Recognition site: GAAGAC(N)2↑ / CTTCTG(N)6↓
  • Источник: Bacillus stearothermophilus V2
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 65
SKU: SE-E297 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E297TS200 uTurbo5 000 u/ml3,300.00 
  • CNY: 257.37 ¥
E298TL1000 uTurbo5 000 u/ml13,200.00 
  • CNY: 1,029.47 ¥
E297S200 uRegular5 000 u/ml3,300.00 
  • CNY: 257.37 ¥
E298L1000 uRegular5 000 u/ml13,200.00 
  • CNY: 1,029.47 ¥

Description

Recognition site and hydrolysis position:

GAAGAC(N)2 GAAGAC(N)2
CTTCTG(N)6 CTTCTG(N)6

Source: Bacillus stearothermophilus V2
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 75 25 25 100 70

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Sinichkina, S.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (2000).
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006