M.HspA I

2,750.00 

  • CNY: 213.29 ¥

DNA Methyltransferase M.HspA I

  • Recognition site: G(5mC)GC /
  • Source: An E.coli strain, that carries the cloned M.HspAI gene from Haemophilus species AI.
  • Reaction buffer: SE-buffer B + SAM
  • Optimal temperature: 37
SKU: M003 Category:

Description

Recognition site
G(5mC)GC
Source An E.coli strain, that carries the cloned M.HspAI gene from Haemophilus species AI.
Description M.HspAI Methyltransferase modifies the internal cytosine residue (C5) in the recognition sequence forming 5`-G(5mC)GC-3`/3`-CG(5mC)G-5`.
Unit definition One unit of the enzyme is the amount required to protect 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 20μl against cleavage by HspAI restriction endonuclease.
Reaction buffer SE-buffer B + SAM
Optimal temperature
37oC
Storage conditions 10 mM Tis-HCl (pH 7.4); 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 100 mM NaCl; 50% glycerol. Store at -20°C.
Notes To obtain 100% activity, SAM should be added to the 1x reaction mix to a final concentration of 0,08 mM.
MSDS: Download MSDS as PDF