Msp I

1,430.00 5,720.00 

  • CNY: 110.55 ¥ - 442.21 ¥

Restriction endonuclease Msp I

  • Recognition site: C↑CGG / GGC↓C
  • Источник: Moraxella species
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E091 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E091TS1000 uTurbo20 000 u/ml1,430.00 
  • CNY: 110.55 ¥
E092TL5000 uTurbo20 000 u/ml5,720.00 
  • CNY: 442.21 ¥
E091S1000 uRegular20 000 u/ml1,430.00 
  • CNY: 110.55 ¥
E092L5000 uRegular20 000 u/ml5,720.00 
  • CNY: 442.21 ¥

Description

Recognition site and hydrolysis position:

CCGG C↑CGG
GGCC GGC↓C

Source: Moraxella species
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 75 50 75 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by methylation: 5`-(5mC)CGG-3`/ 3`-GGC(5mC)-5`.
Notes:
References:
Waalwijk, C., Flavell, R.A., Nucl. Acids Res.5: 3231-3236 (1978).
Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.3, No 4, pp 19-27, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006
M.A. Abdurashitov, V.N. Tomilov, V.A. Chernukhin, D.A. Gonchar, S.Kh. Degtyarev Mammalian chromosomal DNA digestion with restriction endonucleases in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 29-38, 2006