Xba I

2,090.00 8,360.00 

  • CNY: 163.00 ¥ - 652.00 ¥

Restriction endonuclease Xba I

  • Recognition site: T↑CTAGA / AGATC↓T
  • Источник: Из штамма E.coli несущего клонированный ген XbaI из Xanthomonas badrii
  • Оптимальный буфер: SE-буфер O
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E141 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E141S2000 uRegular20 000 u/ml2,090.00 
  • CNY: 163.00 ¥
E142L10000 uRegular20 000 u/ml8,360.00 
  • CNY: 652.00 ¥
E141XS2000 uConcentrated50 000 u/ml2,090.00 
  • CNY: 163.00 ¥
E142XL10000 uConcentrated50 000 u/ml8,360.00 
  • CNY: 652.00 ¥
E141TS2000 uTurbo20 000 u/ml2,090.00 
  • CNY: 163.00 ¥
E142TL10000 uTurbo20 000 u/ml8,360.00 
  • CNY: 652.00 ¥

Description

Recognition site and hydrolysis position:

TCTAGA T↑CTAGA
AGATCT AGATC↓T

Source: An E.coli strain, that carries the cloned gene XbaI from Xanthomonas badrii
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-/HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (dam-/HindIII-digest)
Optimal SE-buffer: SE-buffer O
Enzyme activity (%):

B G O W Y ROSE
75 75 100 50 75 25

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer O, BSA (except E141m, E141T and E142T).
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by overlapping Dam-methylation (GmATC): TCTAGATCNotes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Zain, B,S., Roberts, R.J. J. Mol. Biol. 115: 249-255 (1977).
Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from “Medical genetics” V.6, No 8, pp 29-36, 2007